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High speed liquid chromatography with conventional instruments for the determination of cyclosporine A B C and D in fermentation broth
Summary
Type:
HPLC
Application #:
103670
Detection:
UV, 214nm, 0.2 AUFS
Conditions:
temperature 70°C
eluent: acetonitrile - water - phosphoric acid (700:300:0.1)
flow rate 2.0μl/min
pressure 160bar
Authors:
Kreuzig F
Source:
J Chromatogr 290 (1984) 181 - 186
Details
Legend: Fig. 2. Chromatograms of standard solution (50µg/ml of each cyclosporin) and sample.
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